Nature Biotechnology, Published online: 10 October 2022; doi:10.1038/s41587-022-01508-7We integrated the pre-characterized physical model of super-resolution (SR) microscopy into a deep neural network to guide the denoising of raw images for high-quality SR image reconstruction. This approach enabled us to investigate a wide variety of fragile and rapidly evolving bioprocesses at ultrahigh spatiotemporal resolution over extended imaging times…
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