Nature Biotechnology, Published online: 29 June 2023; doi:10.1038/s41587-023-01858-wTnpB proteins are hypercompact RNA-guided DNA endonucleases. By systematically mining and characterizing TnpB proteins from the IS605 family, we identified potent genome editors and established a framework for high-throughput annotation and screening of TnpB systems in prokaryotic genomes…
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